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Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
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Biochemistry
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JoVE Journal Biochemistry
Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

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1,745 Views

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05:27 min

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July 20, 2022

DOI:

10.3791/63729-v

DOI:
10.3791/63729-v

•

05:27 min
July 20, 2022

•

1 Views
,
1Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III CNIC, 2Centro de Investigaciones Biomédica en Red de Fragilidad y Envejecimiento, Saludable-CIBERFES

Summary

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This protocol describes a comparative assay, using mitochondrial complex activities CI+CIII and CII+CIII in the presence or absence of Na+, to study the existence of partially segmented functional CoQ pools.

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Cite this Article

Hernansanz-Agustín, P., Enríquez, J. A. Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools. J. Vis. Exp. (185), e63729, doi:10.3791/63729 (2022).

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