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Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
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Neuroscience
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JoVE Journal Neuroscience
Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

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2,548 Views

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07:39 min

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April 13, 2022

DOI:

10.3791/63713-v

DOI:
10.3791/63713-v

•

07:39 min
April 13, 2022

•

21 Views
, , , , , ,
1Max-Planck-Institute for Brain Research,Synaptic Plasticity Department, 2Department of Biochemistry and Molecular Biology,Veterinary School, Complutense University of Madrid

Summary

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This protocol describes how to perform cell-type-specific protein labeling with azidonorleucine (ANL) using a mouse line expressing a mutant L274G-Methionine tRNA synthetase (MetRS*) and the necessary steps for labeled cell-type-specific proteins isolation. We outline two possible ANL administration routes in live mice by (1) drinking water and (2) intraperitoneal injections.

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Cite this Article

Nassim-Assir, B., Ouro Corredera, D., Alvarez-Pardo, R., tom Dieck, S., Ciirdaeva, E., Schuman, E. M., Alvarez-Castelao, B. Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line. J. Vis. Exp. (182), e63713, doi:10.3791/63713 (2022).

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