The present protocol details the preparation and visualization of fixed tissue of the Drosophila pupal notum. It can be used for either intact or wounded tissue, and the original architecture of the tissue is preserved. The procedures for dissecting, fixing, and staining are all described in this article.
White, J. S., LaFever, K. S., Page-McCaw, A. Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum. J. Vis. Exp. (182), e63682, doi:10.3791/63682 (2022).