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Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care

Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care

English

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2,339 Views

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11:06 min

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November 11, 2022

DOI:

10.3791/63505-v

DOI:
10.3791/63505-v

•

11:06 min
November 11, 2022

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6 Views
, , , , , , , , , , , , , , , , , , , , , , ,
1Eye Clinic, Department of Ophthalmology,University Hospital Giessen and Marburg GmbH, 2Karl Landsteiner Institute for Retinal Research and Imaging, 3Department of Ophthalmology, University Hospital Kralovske Vinohrady and Third Faculty of Medicine,Charles University in Prague, 4Institute of Macromolecular Chemistry,Czech Academy of Sciences, 5Institute of Animal Physiology and Genetics,Czech Academy of Sciences, 6Department of Cell Biology, Faculty of Science,Charles University, 7Institute of Experimental Medicine,Czech Academy of Sciences, 8Center for Eye Research, Department of Ophthalmology, Oslo University Hospital and Institute of Clinical Medicine, Faculty of Medicine,University of Oslo, 9Moorfields Eye Hospitals UAE, 10Stem Cell Therapies in Neurodegenerative Diseases Lab,Research Center “Principe Felipe”, 11Eye Center Donaustadt, Department of Ophthalmology,Sigmund Freud University

Summary

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The subretinal implantation of retinal pigmented epithelium (RPE) is one of the most promising approaches for the treatment of degenerative retinal diseases. However, the performance of preclinical studies on large-eye animal models remains challenging. This report presents guidelines for the subretinal transplantation of RPE on a cell carrier into minipigs.

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Lytvynchuk, L., Stranak, Z., Studenovska, H., Rais, D., Popelka, Š., Tichotová, L., Nemesh, Y., Kolesnikova, A., Nyshchuk, R., Brymová, A., Ellederová, Z., Čížková, J., Juhásová, J., Juhás, Š., Jendelová, P., Nagymihály, R., Kozak, I., Erceg, S., Binder, S., Müller, B., Stieger, K., Motlik, J., Petrovski, G., Ardan, T. Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care. J. Vis. Exp. (189), e63505, doi:10.3791/63505 (2022).

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