A robust methodology has been developed for conducting in ovo feeding research trials utilizing unincubated commercial broiler eggs to test the ability of natural and synthetic compounds, in this case, nicotinamide riboside, to influence muscle development and growth.
Alcocer, H. M., Xu, X., Gravely, M. E., Gonzalez, J. M. In Ovo Feeding of Commercial Broiler Eggs: An Accurate and Reproducible Method to Affect Muscle Development and Growth. J. Vis. Exp. (175), e63006, doi:10.3791/63006 (2021).