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Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
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June 09, 2021
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Summary
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A headspace solid-phase microextraction-gas-chromatography platform is described here for fast, reliable, and semi-automated volatile identification and quantification in ripe blackcurrant fruits. This technique can be used to increase knowledge about fruit aroma and to select cultivars with enhanced flavor for the purpose of breeding.
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Volatile CompoundsBlackcurrant FruitHeadspace Solid-phase MicroextractionGas Chromatography-mass SpectrometryFruit AromaBreeding ProgramsSemi-automated TechniqueBerry CropsSodium Chloride SolutionCentrifugationInternal StandardGC-MS Auto SamplerHeadspace VialSPME DeviceVolatile DesorptionGC-MS AnalysisRetention TimeMass SpectraKovats Linear Retention IndexPeak AreaInternal StandardBatch Effect Correction
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Pott, D. M., Vallarino, J. G., Osorio, S. Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry. J. Vis. Exp. (172), e62421, doi:10.3791/62421 (2021).
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