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A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
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Neuroscience
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JoVE Journal Neuroscience
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

English

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3,058 Views

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07:56 min

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August 11, 2021

DOI:

10.3791/62127-v

DOI:
10.3791/62127-v

•

07:56 min
August 11, 2021

•

1 Views
, , ,
1Department of Medicine, School of Medicine,Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences,City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics,University of Florida College of Medicine

Summary

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This method introduces a simple technique for the detection of endogenous monoamine release using acute brain slices. The setup uses a 48-well plate containing a tissue holder for monoamine release. Released monoamine is analyzed by HPLC coupled with electrochemical detection. Additionally, this technique provides a screening method for drug discovery.

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Cite this Article

Pino, J. A., Awadallah, N., Norris, A. M., Torres, G. E. A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices. J. Vis. Exp. (174), e62127, doi:10.3791/62127 (2021).

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