The goal of this protocol is to show how to perform an improved assay for mechanical nociception in Drosophila larvae. We use the assay here to demonstrate that mechanical hypersensitivity (allodynia and hyperalgesia) exists in Drosophila larvae.
Lopez-Bellido, R., Galko, M. J. An Improved Assay and Tools for Measuring Mechanical Nociception in Drosophila Larvae. J. Vis. Exp. (164), e61911, doi:10.3791/61911 (2020).