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Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
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Biochemistry
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JoVE Journal Biochemistry
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

English

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9,884 Views

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06:47 min

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January 28, 2021

DOI:

10.3791/61790-v

DOI:
10.3791/61790-v

•

06:47 min
January 28, 2021

•

107 Views
, , , ,
1Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 2School of Biotechnology and Health Sciences,Wuyi University

Summary

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Several methods have been used for analyzing plasma lipoproteins; however, ultracentrifugation is still one of the most popular and reliable methods. Here, we describe a method regarding how to isolate lipoproteins from plasma using sequential density ultracentrifugation and how to analyze the apolipoproteins for both diagnostic and research purposes.

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Cite this Article

Niimi, M., Yan, H., Chen, Y., Wang, Y., Fan, J. Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation. J. Vis. Exp. (167), e61790, doi:10.3791/61790 (2021).

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