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Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection
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January 21, 2021
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1Department of Biomedical Engineering,University of Minnesota, 2Department of Biomedical Engineering,Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering,University of Minnesota, 6Graduate Program in Neuroscience,University of Minnesota
Summary
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This protocol demonstrates the use of a robotic platform for microinjection into single neural stem cells and neurons in brain slices. This technique is versatile and offers a method of tracking cells in tissue with high spatial resolution.
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Shull, G., Haffner, C., Huttner, W. B., Taverna, E., Kodandaramaiah, S. B. Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection. J. Vis. Exp. (167), e61599, doi:10.3791/61599 (2021).
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