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Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
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Immunology and Infection
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JoVE Journal Immunology and Infection
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

English

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1,507 Views

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06:06 min

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September 11, 2020

DOI:

10.3791/61594-v

DOI:
10.3791/61594-v

•

06:06 min
September 11, 2020

•

2 Views
, , , ,
1Faculty of Infections and Tropical Diseases, Department of Infection Biology,London School of Hygiene and Tropical Medicine, 2Division of Medicine,University College London, 3Division of Infection and Immunity,University College London UCL

Summary

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Here, we describe a S. pneumoniae serotype 1 strain 519/43 that can be genetically modified by using its ability to naturally acquire DNA and a suicide-plasmid. As proof of principle, an isogenic mutant in the pneumolysin (ply) gene was made.

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Cite this Article

Terra, V. S., Plumptre, C. D., Wall, E. C., Brown, J. S., Wren, B. W. Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43. J. Vis. Exp. (163), e61594, doi:10.3791/61594 (2020).

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