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Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

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3,323 Views

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08:44 min

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July 30, 2020

DOI:

10.3791/61585-v

DOI:
10.3791/61585-v

•

08:44 min
July 30, 2020

•

44 Views
, ,
1Robert M. Berne Cardiovascular Research Center,University of Virginia, 2Department of Pathology,University of Virginia, 3Department of Medicine, Division of Cardiovascular Medicine,University of Virginia

Summary

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The goal of this protocol is to synthesize fluorescently-labeled liposomes and use flow cytometry to identify in vivo localization of liposomes at a cellular level.

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Cite this Article

Osinski, V., Klibanov, A. L., McNamara, C. A. Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes. J. Vis. Exp. (161), e61585, doi:10.3791/61585 (2020).

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