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Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

English

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4,485 Views

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07:06 min

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May 14, 2020

DOI:

10.3791/61161-v

DOI:
10.3791/61161-v

•

07:06 min
May 14, 2020

•

22 Views
, , ,
1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II,U.S. Food and Drug Administration

Summary

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Presented here is a protocol for the primary clarification of CHO cell culture using an acoustic separator. This protocol can be used for the primary clarification of shake flask cultures or bioreactor harvests and has the potential application for continuous clarification of the cell bleed material during perfusion bioreactor operations.

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Cite this Article

Hong, J. S., Azer, N., Agarabi, C., Fratz-Berilla, E. J. Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator. J. Vis. Exp. (159), e61161, doi:10.3791/61161 (2020).

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