Here we describe a method for the generation of germ-free chicks from eggs of a commercial broiler line, the Ross PM3. This method can be adapted for the generation of germ-free animals from other poultry species.
Guitton, E., Faurie, A., Lavillatte, S., Chaumeil, T., Gaboriaud, P., Bussière, F., Laurent, F., Lacroix-Lamandé, S., Guabiraba, R., Schouler, C. Production of Germ-Free Fast-Growing Broilers from a Commercial Line for Microbiota Studies. J. Vis. Exp. (160), e61148, doi:10.3791/61148 (2020).