Journal
/
Immunology and Infection
/
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

English

Automatically Generated

5,142 Views

•

09:25 min

•

May 24, 2020

DOI:

10.3791/61049-v

DOI:
10.3791/61049-v

•

09:25 min
May 24, 2020

•

1 Views
, , , ,
1Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, 2Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Summary

Automatically generated

This is a method to generate “scarless” recombinant vaccinia viruses using host-range selection and visual identification of recombinant viruses.

Explore More Videos

Recombinant PoxvirusesHost Range SelectionFluorescent MarkersHomologous RecombinationTransient Dominant SelectionVaccinia VirusForeign Gene ExpressionVaccinesPCRDNA Gel ExtractionDNA AssemblyE. Coli Transformation

Read Article

Cite this Article

Vipat, S., Brennan, G., Park, C., Haller, S. L., Rothenburg, S. Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection. J. Vis. Exp. (159), e61049, doi:10.3791/61049 (2020).

Download .ris file

Copy

Share Video

.

Copy