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Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
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Immunology and Infection
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JoVE Journal Immunology and Infection
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate

Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate

English

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8,180 Views

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09:06 min

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February 22, 2020

DOI:

10.3791/60466-v

DOI:
10.3791/60466-v

•

09:06 min
February 22, 2020

•

5 Views
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1Department of Biomedical Sciences, Joan C. Edwards School of Medicine,Marshall University, 2Progenesis Technologies LLC,Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine,Marshall University

Summary

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Here, we describe a growth condition to culture the small colony variant of Pseudomonas aeruginosa. We also describe two separate methods for the detection and quantitation of the exopolysaccharide alginate produced by P. aeruginosa using a traditional uronic acid carbazole assay and an alginate-specific monoclonal antibody (mAb) based ELISA.

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Al Ahmar, R., Kirby, B. D., Yu, H. D. Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate. J. Vis. Exp. (156), e60466, doi:10.3791/60466 (2020).

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