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Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
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Biochemistry
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JoVE Journal Biochemistry
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

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6,402 Views

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08:09 min

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October 15, 2019

DOI:

10.3791/60369-v

DOI:
10.3791/60369-v

•

08:09 min
October 15, 2019

•

1 Views
,
1Department of Biological Sciences,Smith College, 2Center for Microscopy and Imaging,Smith College

Summary

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The goal of this protocol is to form ensembles of molecular motors on DNA origami nanostructures and observe the ensemble motility using total internal reflection fluorescence microscopy.

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Cite this Article

Hu, J., Derr, N. D. Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation. J. Vis. Exp. (152), e60369, doi:10.3791/60369 (2019).

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