The goal of this protocol is to isolate microvessels from multiple regions of the central nervous system of lissencephalic and gyrencephalic vertebrates.
Yuan, Y., Dayton, J. R., Freese, M., Dorflinger, B. G., Cruz-Orengo, L. Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups. J. Vis. Exp. (155), e60291, doi:10.3791/60291 (2020).