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Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

English

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7,366 Views

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07:25 min

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December 17, 2019

DOI:

10.3791/60015-v

DOI:
10.3791/60015-v

•

07:25 min
December 17, 2019

•

9 Views
,
1Department of Medicine,Washington University School of Medicine, 2Department of Immunology and Pathology,Washington University School of Medicine, 3Department of Developmental Biology,Washington University School of Medicine

Summary

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Presented here is a protocol to isolate various subsets of macrophages and other non-immune cells from human and mouse myocardium by preparing a single cell suspension through enzymatic digestion. Gating schemes for flow cytometry based identification and characterization of isolated macrophages are also presented.

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Cite this Article

Bajpai, G., Lavine, K. J. Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens. J. Vis. Exp. (154), e60015, doi:10.3791/60015 (2019).

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