Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

Robert  S. Brzozowski; Maria  L. White; Prahathees J. Eswara

doi:10.3791/59905

/

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

6,740 Views

•

05:57 min

•

November 23, 2019

DOI:

10.3791/59905-v

DOI:

10.3791/59905-v

•

05:57 min

November 23, 2019

•

7 Views

Robert S. Brzozowski¹, Maria L. White¹, Prahathees J. Eswara¹

¹Department of Cell Biology, Microbiology and Molecular Biology,University of South Florida

Summary

This article provides a step-by-step guide to investigate protein subcellular localization dynamics and to monitor morphological changes using high-resolution fluorescence microscopy in Bacillus subtilis and Staphylococcus aureus.

Explore More Videos

Live-cell Fluorescence Microscopy Subcellular Protein Localization Cell Morphology Changes Bacteria Sample Preparation Membrane Staining Dye Agarose Slab High-resolution Deconvolution Microscope 100x Oil Immersion Objective Resolve 3D Imaging Software Z-stacks Sample Thickness

Read Article

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

Automatically Generated

Summary

Automatically generated

Explore More Videos