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Purification of High Yield Extracellular Vesicle Preparations Away from Virus
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Immunology and Infection
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JoVE Journal Immunology and Infection
Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

English

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11,052 Views

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00:07 min

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September 12, 2019

DOI:

10.3791/59876-v

DOI:
10.3791/59876-v

•

00:07 min
September 12, 2019

•

7 Views
, , , , , , , ,
1Laboratory of Molecular Virology, School of Systems Biology,George Mason University, 2American Type Culture Collection (ATCC), 3ATCC Cell Systems, 4Ceres Nanosciences, Inc, 5Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine,Florida International University

Summary

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This protocol isolates extracellular vesicles (EVs) away from virions with high efficiency and yield by incorporating EV precipitation, density gradient ultracentrifugation, and particle capture to allow for a streamlined workflow and a reduction of starting volume requirements, resulting in reproducible preparations for use in all EV research.

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Cite this Article

DeMarino, C., Barclay, R. A., Pleet, M. L., Pinto, D. O., Branscome, H., Paul, S., Lepene, B., El-Hage, N., Kashanchi, F. Purification of High Yield Extracellular Vesicle Preparations Away from Virus. J. Vis. Exp. (151), e59876, doi:10.3791/59876 (2019).

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