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Use of Alu Element Containing Minigenes to Analyze Circular RNAs
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Biochemistry
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JoVE Journal Biochemistry
Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

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7,169 Views

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13:10 min

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March 10, 2020

DOI:

10.3791/59760-v

DOI:
10.3791/59760-v

•

13:10 min
March 10, 2020

•

2 Views
, , ,
1Institute for Molecular and Cellular Biochemistry,University of Kentucky

Summary

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We clone and analyze reporter genes generating circular RNAs. These reporter genes are larger than constructs to analyze linear splicing and contain Alu elements. To investigate the circular RNAs, the constructs are transfected into cells and resulting RNA is analyzed using RT-PCR after removal of linear RNA.

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Cite this Article

Welden, J. R., Pawluchin, A., van Doorn, J., Stamm, S. Use of Alu Element Containing Minigenes to Analyze Circular RNAs. J. Vis. Exp. (157), e59760, doi:10.3791/59760 (2020).

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