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Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

English

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8,469 Views

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12:15 min

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May 29, 2019

DOI:

10.3791/59734-v

DOI:
10.3791/59734-v

•

12:15 min
May 29, 2019

•

2 Views
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1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas,Universidade de São Paulo, 2Departamento de Farmacociências,Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental – LIM05, Hospital das Clínicas, Faculdade de Medicina,Universidade de São Paulo, 4Instituto de Estudos Avançados,Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química,Universidade de São Paulo

Summary

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We describe here methods for sensitive and accurate quantification of the lesions 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 1,N6-etheno-2'-deoxyadenosine (1,N6-dAdo) and 1,N2-etheno-2'-deoxyguanosine (1,N2-dGuo) in DNA. The methods were applied to the assessment of the effects of ambient fine particulate matter (PM2.5) in tissues (lung, liver and kidney) of exposed A/J mice.

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DNA LesionsMass SpectrometryOxidative StressHPLC-MS/MSEtheno AdductsDNA QuantificationSample Preparation

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Franco de Oliveira, T., Falcão de Oliveira, A. A., Lemos, M., Veras, M., Saldiva, P. H. N., Gennari de Medeiros, M. H., Di Mascio, P., de Melo Loureiro, A. P. Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter. J. Vis. Exp. (147), e59734, doi:10.3791/59734 (2019).

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