/
/
Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
A subscription to JoVE is required to view this content. Sign in or start your free trial.
8,152 Views
•
07:00 min
•
June 04, 2019
DOI:
Summary
Automatically generated
Presented here is a protocol for measuring proliferating CD4+ T cells in response to antigenic proteins or peptides using dye dilution. This assay is particularly sensitive to rare antigen-specific T cells and can be modified to facilitate cloning of antigen-specific cells.
Read Article
Cite this Article
Di Carluccio, A. R., Tresoldi, E., So, M., Mannering, S. I. Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester. J. Vis. Exp. (148), e59545, doi:10.3791/59545 (2019).
Copy