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Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

English

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7,034 Views

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10:25 min

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June 12, 2019

DOI:

10.3791/59496-v

DOI:
10.3791/59496-v

•

10:25 min
June 12, 2019

•

2 Views
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1Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Summary

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Brush cells are rare cholinergic chemosensory epithelial cells found in the naïve mouse trachea. Due to their limited numbers, ex vivo evaluation of their functional role in airway immunity and remodeling is challenging. We describe a method for isolation of tracheal brush cells by flow cytometry.

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Cite this Article

Ualiyeva, S., Yoshimoto, E., Barrett, N. A., Bankova, L. G. Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas. J. Vis. Exp. (148), e59496, doi:10.3791/59496 (2019).

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