Journal
/
Biochemistry
/
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

English

Automatically Generated

7,831 Views

•

08:37 min

•

June 25, 2019

DOI:

10.3791/59308-v

DOI:
10.3791/59308-v

•

08:37 min
June 25, 2019

•

1 Views
, , , ,
1Department of Sciences and Technologies (DST),University of Sannio, 2Endocannabinoid Research Group,Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions,University of Naples Federico II

Summary

Automatically generated

Presented here are protocols for immunohistochemical characterization and localization of orexin peptide, orexin receptors, and endocannabinoid receptors in the gut and brains of normal and diet-induced obesity (DIO) adult zebrafish models using immunoperixidase and double immunofluorescence methods.

Explore More Videos

ImmunohistochemistryImmunoperoxidaseImmunofluorescenceZebrafishOrexinEndocannabinoid ReceptorsCryosectioningBlockingPrimary AntibodiesIncubation

Read Article

Cite this Article

Imperatore, R., D'Angelo, L., De Girolamo, P., Cristino, L., Paolucci, M. Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods. J. Vis. Exp. (148), e59308, doi:10.3791/59308 (2019).

Download .ris file

Copy

Share Video

.

Copy