Journal
/
Immunology and Infection
/
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

English

Automatically Generated

10,340 Views

•

08:07 min

•

January 07, 2019

DOI:

10.3791/58621-v

DOI:
10.3791/58621-v

•

08:07 min
January 07, 2019

•

21 Views
, ,
1Division of Gastroenterology and Hepatology, Department of Medicine,University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology,University of Colorado Anschutz Medical Campus

Summary

Automatically generated

The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.

Explore More Videos

LiverLymphatic Endothelial CellsFlow CytometrySingle-cell SuspensionLiver Biology

Read Article

Cite this Article

Finlon, J. M., Burchill, M. A., Tamburini, B. A. J. Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells. J. Vis. Exp. (143), e58621, doi:10.3791/58621 (2019).

Download .ris file

Copy

Share Video

.

Copy