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Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

English

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20,810 Views

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08:13 min

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January 20, 2019

DOI:

10.3791/58595-v

DOI:
10.3791/58595-v

•

08:13 min
January 20, 2019

•

12 Views
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1Inovio Pharmaceuticals, Inc, 2Mycotic and Parasitic Agents and Mycobacteria,Robert Koch Institute

Summary

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The development of the interferon-gamma ELISpot assay for guinea pig PBMCs allows the characterization of T-cell responses in this highly relevant model for studying infectious diseases. We have applied the assay to measure T cell responses associated with intradermal delivery of DNA vaccines.

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Schultheis, K., Schaefer, H., Pugh, H. M., Yung, B. S., Oh, J., Nguyen, J., Humeau, L., Broderick, K. E., Smith, T. R. Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination. J. Vis. Exp. (143), e58595, doi:10.3791/58595 (2019).

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