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High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

English

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7,597 Views

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10:07 min

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January 07, 2019

DOI:

10.3791/58351-v

DOI:
10.3791/58351-v

•

10:07 min
January 07, 2019

•

1 Views
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1Department of Microbial Infection and Immunity,The Ohio State University, 2Department of Microbiology,The Ohio State University, 3Infectious Diseases Institute,The Ohio State University, 4Division of Biostatistics, College of Public Health,The Ohio State University

Summary

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Here we describe a high-throughput fluorescence-based assay that measures the plasma membrane resealing efficiency through fluorometric and imaging analyses in living cells. This assay can be used for screening drugs or target genes that regulate plasma membrane resealing in mammalian cells.

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Cite this Article

Lam, J. G., Song, C., Seveau, S. High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells. J. Vis. Exp. (143), e58351, doi:10.3791/58351 (2019).

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