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Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee
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Biochemistry
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JoVE Journal Biochemistry
Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

English

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7,608 Views

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08:30 min

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September 14, 2018

DOI:

10.3791/58173-v

DOI:
10.3791/58173-v

•

08:30 min
September 14, 2018

•

2 Views
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1Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology, 2Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Summary

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To test the inhibitory effects of pharmacologic agents on phospholipase C (PLC) in different regions of the honeybee brain, we present a biochemical assay to measure PLC activity in those regions. This assay could be useful for comparing PLC activity among tissues, as well as among bees exhibiting different behaviors.

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Cite this Article

Suenami, S., Miyazaki, R., Kubo, T. Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee. J. Vis. Exp. (139), e58173, doi:10.3791/58173 (2018).

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