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Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

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8,250 Views

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07:21 min

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July 29, 2018

DOI:

10.3791/58061-v

DOI:
10.3791/58061-v

•

07:21 min
July 29, 2018

•

5 Views
,
1Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 2Research Division of Immunology,Cedars-Sinai Medical Center

Summary

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We demonstrate how to identify and isolate 6 subsets of myeloid progenitors from murine bone marrow using a combination of magnetic and fluorescence sorting (MACS and FACS). This protocol can be used for in vitro culture assays (methylcellulose or liquid cultures), in vivo adoptive transfer experiments, and RNA/protein analyses.

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Myeloid ProgenitorsBone MarrowMACSLineage-negative CellsFACSNeutrophilsMonocytesDendritic CellsCell IsolationHematologyImmunology

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Yáñez, A., Goodridge, H. S. Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow. J. Vis. Exp. (137), e58061, doi:10.3791/58061 (2018).

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