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Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae
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Neuroscience
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JoVE Journal Neuroscience
Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

English

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8,437 Views

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10:29 min

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June 02, 2018

DOI:

10.3791/57485-v

DOI:
10.3791/57485-v

•

10:29 min
June 02, 2018

•

8 Views
,
1Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics,SOKENDAI (The Graduate University for Advanced Studies)

Summary

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Here, we present a protocol to ablate a genetically labeled subpopulation of neurons by a two-photon laser from Zebrafish larvae.

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Muto, A., Kawakami, K. Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae. J. Vis. Exp. (136), e57485, doi:10.3791/57485 (2018).

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