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Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa
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Immunology and Infection
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JoVE Journal Immunology and Infection
Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa

Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa

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5,397 Views

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07:27 min

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July 12, 2018

DOI:

10.3791/57447-v

DOI:
10.3791/57447-v

•

07:27 min
July 12, 2018

•

1 Views
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1Department of Biochemistry and Molecular Biology,University of South Alabama, 2Department of Physiology and Cell Biology,University of South Alabama, 3Department of Chemical and Biomolecular Engineering,University of South Alabama, 4Center for Lung Biology,University of South Alabama

Summary

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Simple methods are described for demonstrating the production of cytotoxic amyloids following infection of pulmonary endothelium by Pseudomonas aeruginosa.

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Cytotoxic AmyloidsPseudomonas AeruginosaPulmonary Endothelial CellsCytotoxicitySupernatantCell MonolayerCell CultureMicroscopyImageJPulmonary CareHospital-acquired Pneumonia

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Balczon, R., Francis, M., Leavesley, S., Stevens, T. Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa. J. Vis. Exp. (137), e57447, doi:10.3791/57447 (2018).

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