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A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae
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Behavior
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JoVE Journal Behavior
A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae

A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae

English

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7,423 Views

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09:04 min

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March 27, 2018

DOI:

10.3791/57131-v

DOI:
10.3791/57131-v

•

09:04 min
March 27, 2018

•

3 Views
, ,
1Department of Biosciences,Rice University, 2Yale Medical School

Summary

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The protocol describes a simple assay to identify Drosophila melanogaster larvae that are experiencing hypoxia under normal atmospheric oxygen levels. This protocol allows hypoxic larvae to be distinguished from other mutants that show overlapping phenotypes such as sluggishness or slow growth.

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Cite this Article

Qiang, K. M., Zhou, F., Beckingham, K. M. A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae. J. Vis. Exp. (133), e57131, doi:10.3791/57131 (2018).

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