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Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
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JoVE Journal Genetics
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

English

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12,027 Views

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11:12 min

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February 22, 2018

DOI:

10.3791/56693-v

DOI:
10.3791/56693-v

•

11:12 min
February 22, 2018

•

12 Views
, , , ,
1Department of Biotechnology and Animal Science,National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences,Chonbuk National University, 3Department of Entomology,National Taiwan University

Summary

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This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells.

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Transient Gene ExpressionInsect CellsSf9Protein Functional AssayAnti-apoptotic ActivityHeat Shock InductionProtein ExpressionCell CultureTransfectionIAP3

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Chang, J., Lee, S. J., Kim, J. S., Wang, C., Nai, Y. Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay. J. Vis. Exp. (132), e56693, doi:10.3791/56693 (2018).

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