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Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature
JoVE Journal
Medicine
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JoVE Journal Medicine
Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

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12,525 Views

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10:07 min

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December 26, 2017

DOI:

10.3791/56674-v

DOI:
10.3791/56674-v

•

10:07 min
December 26, 2017

•

5 Views
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1Department of Vascular Biology and Tumorangiogenesis, Center for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim,Heidelberg University, 2V. Medical Clinic, Medical Faculty Mannheim,Heidelberg University

Summary

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Here, we discuss a method protocol which will allow an easy analysis of the adult tg(fli:EGFP) zebrafish retinal vasculature as a fast read-out in settings of long-term vascular pathologies linked to neoangiogenesis and structural changes.

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Wiggenhauser, L. M., Kohl, K., Dietrich, N., Hammes, H., Kroll, J. Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature. J. Vis. Exp. (130), e56674, doi:10.3791/56674 (2017).

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