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In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

English

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9,685 Views

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07:05 min

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November 28, 2017

DOI:

10.3791/56255-v

DOI:
10.3791/56255-v

•

07:05 min
November 28, 2017

•

2 Views
, , ,
1Department of Melanoma Medical Oncology,University of Texas MD Anderson Cancer Center, 2Department of Immunology,University of Texas MD Anderson Cancer Center, 3Department of Radiation Oncology,The Second Hospital of Jilin University

Summary

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The goal of this protocol is to allow for detection of in vivo antigen-specific killing of a target cell in a murine model.

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Cite this Article

Haymaker, C. L., Hailemichael, Y., Yang, Y., Nurieva, R. In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model. J. Vis. Exp. (129), e56255, doi:10.3791/56255 (2017).

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