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A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

English

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22,959 Views

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06:08 min

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August 09, 2017

DOI:

10.3791/56191-v

DOI:
10.3791/56191-v

•

06:08 min
August 09, 2017

•

26 Views
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1UCLA Immunogenetics Center, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine,University of California, Los Angeles

Summary

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A flow cytometry-based method to quantitatively determine the cytotoxic activity of human natural killer cells is shown here.

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Flow CytometryCytotoxicity AssayNK Cell ActivityNK Cell DeficiencyPBMCK562 CellsCFSEEffector-to-target Cell Ratio

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Cite this Article

Kandarian, F., Sunga, G. M., Arango-Saenz, D., Rossetti, M. A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity. J. Vis. Exp. (126), e56191, doi:10.3791/56191 (2017).

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