Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Rizwan Rehimi; Michaela Bartusel; Francesca Solinas; Janine Altm&#252;ller; Alvaro Rada-Iglesias

doi:10.3791/56186

Journal

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Genetics

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Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

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Genetics

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JoVE Journal Genetics

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

15,337 Views

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12:47 min

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August 29, 2017

DOI:

10.3791/56186-v

DOI:

10.3791/56186-v

•

12:47 min

August 29, 2017

•

15 Views

Rizwan Rehimi¹, Michaela Bartusel¹, Francesca Solinas², Janine Altmüller², Alvaro Rada-Iglesias^1,3

¹Center for Molecular Medicine Cologne (CMMC),University of Cologne, ²Cologne Center for Genomics (CCG),University of Cologne, ³Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), Research Center and Systems Biology of Ageing Cologne,University of Cologne

Summary

Here, we describe a chromatin immunoprecipitation (ChIP) and ChIP-seq library preparation protocol to generate global epigenomic profiles from low-abundance chicken embryonic samples.

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Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

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