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Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment
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Immunology and Infection
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JoVE Journal Immunology and Infection
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

English

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9,961 Views

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08:22 min

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July 19, 2017

DOI:

10.3791/56122-v

DOI:
10.3791/56122-v

•

08:22 min
July 19, 2017

•

5 Views
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1Pathology Division,United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 2Diagnostic Systems Division,United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 3Microscopy Innovations LLC

Summary

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This protocol provides instruction for negative staining virus samples which can easily be used in BSL-2, -3, or -4 laboratories. It includes the use of an innovative processing capsule, which protects the transmission electron microscopy grid and provides the user easier handling in the more turbulent environments within biocontainment.

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Cite this Article

Blancett, C. D., Monninger, M. K., Nguessan, C. A., Kuehl, K. A., Rossi, C. A., Olschner, S. P., Williams, P. L., Goodman, S. L., Sun, M. G. Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment. J. Vis. Exp. (125), e56122, doi:10.3791/56122 (2017).

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