/
/
A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
A subscription to JoVE is required to view this content. Sign in or start your free trial.
7,854 Views
•
07:11 min
•
December 30, 2017
DOI:
Summary
Automatically generated
The ability to accurately detect transcripts or proteins in Drosophila tissues is critical for studying their abundance and localization related to the development process. Here is the description of a straightforward procedure to dissect pupal wings. These wings can be used as samples in several techniques (immunohistochemistry, PCR assay, etc.).
Read Article
Cite this Article
Bolatto, C., Parada, C., Colmenares, V. A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays. J. Vis. Exp. (130), e55854, doi:10.3791/55854 (2017).
Copy