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Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
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Developmental Biology
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JoVE Journal Developmental Biology
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

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9,287 Views

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08:28 min

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June 30, 2017

DOI:

10.3791/55818-v

DOI:
10.3791/55818-v

•

08:28 min
June 30, 2017

•

2 Views
, , ,
1Department of Large Animal Clinical Sciences,Michigan State University, 2Department of Pharmacology and Toxicology,Michigan State University

Summary

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Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).

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Cite this Article

Thelen, K., Ayala-Lopez, N., Watts, S. W., Contreras, G. A. Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting. J. Vis. Exp. (124), e55818, doi:10.3791/55818 (2017).

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