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Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
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Genetics
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JoVE Journal Genetics
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

English

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7,726 Views

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08:56 min

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June 27, 2017

DOI:

10.3791/55505-v

DOI:
10.3791/55505-v

•

08:56 min
June 27, 2017

•

2 Views
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1Division of Nephrology, First Department of Integrated Medicine,Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University

Summary

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Here we present a protocol for the detection of microRNA expression in rat peritoneal membrane using quantitative real-time reverse-transcription polymerase chain reaction. This method is suitable for studying the microRNA expression profile in rat peritoneal membrane in several pathological conditions.

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Keyword Extraction:MiRNAPeritoneal MembraneQuantitative Real-time PCRRNA PurificationRatHomogenizationPhenol-guanidineChloroformEthanolSpin Column

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Hirai, K., Yoshizawa, H., Imai, T., Igarashi, Y., Hirahara, I., Ookawara, S., Ishibashi, K., Morishita, Y. Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR. J. Vis. Exp. (124), e55505, doi:10.3791/55505 (2017).

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