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Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
JoVE Journal
Medicine
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JoVE Journal Medicine
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

English

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10,754 Views

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10:35 min

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December 03, 2017

DOI:

10.3791/55147-v

DOI:
10.3791/55147-v

•

10:35 min
December 03, 2017

•

3 Views
, , , , , , ,
1Institute of Medical Microbiology, Immunology and Parasitology,University Hospital Bonn, 2Department of Orthopaedics and Trauma Surgery,University Hospital Bonn, 3Division of EU Cooperation/Microbiology,Paul-Ehrlich-Institute

Summary

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Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.

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Revision ArthroplastyImplant SonicationMultiplex Polymerase Chain ReactionPeri-prosthetic Joint InfectionJoint AspirationMicrobiological ResultsPathogen DetectionDifferential DiagnosticsJoint Fluid AspirationBlood Culture FlaskPCR AnalysisCell CountEDTA

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Hischebeth, G. T., Gravius, S., Buhr, J. K., Molitor, E., Wimmer, M. D., Hoerauf, A., Bekeredjian-Ding, I., Randau, T. M. Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction. J. Vis. Exp. (130), e55147, doi:10.3791/55147 (2017).

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