Journal
/
Developmental Biology
/
In Vitro Differentiation of Mature Myofibers for Live Imaging
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
In Vitro Differentiation of Mature Myofibers for Live Imaging

In Vitro Differentiation of Mature Myofibers for Live Imaging

English

Automatically Generated

9,889 Views

•

08:12 min

•

January 07, 2017

DOI:

10.3791/55141-v

DOI:
10.3791/55141-v

•

08:12 min
January 07, 2017

•

4 Views
, , ,
1Instituto de Medicina Molecular, Faculdade de Medicina,Universidade de Lisboa, 2Myology Research Center, UM76-INSERM U974-CNRS FRE 361,Sorbonne University, UPMC University of Paris 6

Summary

Automatically generated

Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.

Explore More Videos

In Vitro DifferentiationMature MyofibersLive ImagingNeonatal Mouse MyoblastsTransverse Shear TriadSarcomere AssemblyMyofibril AssemblyOrganelle PositioningTime-lapse MicroscopyEnzymatic DigestionCell IsolationFibroblast AdhesionBasement Membrane Coating

Read Article

Cite this Article

Pimentel, M. R., Falcone, S., Cadot, B., Gomes, E. R. In Vitro Differentiation of Mature Myofibers for Live Imaging. J. Vis. Exp. (119), e55141, doi:10.3791/55141 (2017).

Download .ris file

Copy

Share Video

.

Copy