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A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

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19,013 Views

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09:45 min

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January 25, 2017

DOI:

10.3791/54771-v

DOI:
10.3791/54771-v

•

09:45 min
January 25, 2017

•

8 Views
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1Interactions Arbres – Microorganismes, UMR1136,INRA Université de Lorraine, 2Ecologie et Ecophysiologie Forestières – PTEF, UMR 1137,INRA Université de Lorraine, 3Biosciences Division,Oak Ridge National Laboratory

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This protocol describes a new method to grow and qualitatively analyze bacterial biofilms on fungal hyphae by confocal and electron microscopy.

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Bacterial BiofilmFilamentous Fungal ColoniesConfocal MicroscopyElectron MicroscopyMulti-scale AnalysisCellophane MembraneEDTAFungal Pre-cultureP. FluorescensPotassium Phosphate Buffer

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Miquel Guennoc, C., Rose, C., Guinnet, F., Miquel, I., Labbé, J., Deveau, A. A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy. J. Vis. Exp. (119), e54771, doi:10.3791/54771 (2017).

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