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Immunology and Infection
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Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

English

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10,690 Views

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11:50 min

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November 04, 2016

DOI:

10.3791/54698-v

DOI:
10.3791/54698-v

•

11:50 min
November 04, 2016

•

4 Views
, , ,
1Department of Comparative Biomedical Sciences,The Royal Veterinary College, 2Institute of Physiology I, Cardiology & Vascular Medicine,Eberhard Karls University of Tuebingen, 3Department of Clinical Science and Services,The Royal Veterinary College

Summary

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Many experimental systems have been utilized to understand the mechanisms regulating T cell development and function in an immune response. Here a genetic approach using retroviral transduction is described, which is economic, time efficient, and most importantly, highly informative in identifying regulatory pathways.

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Cite this Article

Singh, Y., Garden, O. A., Lang, F., Cobb, B. S. Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function. J. Vis. Exp. (117), e54698, doi:10.3791/54698 (2016).

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