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Immunology and Infection
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A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice

A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice

English

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8,790 Views

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07:43 min

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September 28, 2016

DOI:

10.3791/54508-v

DOI:
10.3791/54508-v

•

07:43 min
September 28, 2016

•

5 Views
,
1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences (NIEHS),National Institutes of Health

Summary

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Several methods have been described in the literature for modeling bacterial pneumonia in mice. Herein, we describe a non-invasive, inexpensive, rapid method for inducing pneumonia via aspiration (i.e., inhalation) of a bacterial inoculum pipetted into the oropharynx. Downstream methods for assessment of the pulmonary innate immune response are also detailed.

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Madenspacher, J. H., Fessler, M. B. A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice. J. Vis. Exp. (115), e54508, doi:10.3791/54508 (2016).

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