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Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

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7,532 Views

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11:39 min

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April 29, 2016

DOI:

10.3791/53792-v

DOI:
10.3791/53792-v

•

11:39 min
April 29, 2016

•

1 Views
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1CNRS UMR8197 – INSERM U1024,IBENS, Institut de Biologie de l’École Normale Supérieure, 2Department of Physiology Development and Neuroscience,University of Cambridge

Summary

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Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.

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Cite this Article

Giger, F. A., Dumortier, J. G., David, N. B. Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos. J. Vis. Exp. (110), e53792, doi:10.3791/53792 (2016).

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