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Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
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Immunology and Infection
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JoVE Journal Immunology and Infection
Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

English

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9,420 Views

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08:34 min

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March 02, 2016

DOI:

10.3791/53682-v

DOI:
10.3791/53682-v

•

08:34 min
March 02, 2016

•

1 Views
,
1Department of Medicine, Division of Immunobiology,University of Vermont, 2Department of Microbiology & Molecular Genetics,University of Vermont

Summary

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The first step in the arenavirus life cycle is attachment of viral particles to host cells. We report a quantitative (q)RT-PCR-based assay for ultrasensitive detection and quantitation of arenavirus attachment events.

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Cite this Article

Klaus, J. P., Botten, J. Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment. J. Vis. Exp. (109), e53682, doi:10.3791/53682 (2016).

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